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NGS-QC Team Posted Subject : Welcome to NGS-QC Portal Posted Date
Apr 24, 2014
Joined Date
Apr 17, 2014
As you certainly know datasets obtained by enrichment-based technologies coupled to massive parallel sequencing (ChIP-seq, DNase-seq, FAIRE-seq, GRO-seq, MeDIP-seq, MRE-seq, …) can vary significantly, even in cases where for example the same cell types and antibodies are used for ChIP-seq. The complexity of these assays, differences in conditions (crosslinking, chromatin shearing, …) and variation in experimenter manipulation are aspects that contribute to these apparent variation. Our proprietary NGS-QC system represents the first universal solution for assessing quality descriptors for such kind of datasets, covers the largest collection of certified publicly available datasets and is freely available to the academic community. We are working further to develop this tool and expand the database to ultimately harbour all enrichment-based NGS profiles, such that the scientific community may count with a comprehensive resource of quality certified datasets. Furthermore, the NGS-QC Generator tool allows you to assess the quality of your own generated datasets, thus to decide at an early time point to continue the analysis or optimize conditions. The NGS-QC report can also help you to choose a sequencing depth to use to obtain a certain quality level. We invite all users to share their experience in using these tools, as well as your thoughts concerning the necessity of having quality descriptors for ChIP-seq and enrichment-related datasets, through this dedicated community forum. Best regards, The NGS-QC team of the Gronemeyer Lab